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KMID : 0366119740020020095
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Korean Journal of Applied Microbiology & Bioengineering
1974 Volume.2 No. 2 p.95 ~ p.101
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Studies on the Production of Yeast. (Part 1)
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Abstract
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Studies on the optimum conditions of acid hydrolysis of sweet potato starch cake and its utilization on the production of Saccharomyces cerevisiae as a carbon source were conducted and the results showed as follows;
1. The highest hydrolysis rate, 62.7% of the reducing sugar based on the weight of the dry matter, was obtained when the starch cake was hydrolyzed with 1.0% of hydrochloric acid at 2.0 kg/㎠ for 30 minutes.
2. But the yeast grew most favorably on the hydrolyzate obtained by treating the starch cake with 0.5% of hydrochloric acid at 2.0 kg/㎠ for 10 minutes. Reducing sugar content of hydrolyzate was 51.49%,
3. The optimum pH of the culture medium was 7. 0, Cell growth reached to the maximum at 36 hours of cultivation time.
4. According to the vitamin requirement tests, Ca-pantothenate was found to be a promoting factor for the growth of the yeast cells.
5. "Gluten acid hydrolyzate" was most effective to the cell growth when added to the medium at the concentration of 0.1 % as a nitrogen source.
6. Sacch. cerevisiae could assimilate the sugars in the hydrolyzate about 89.1%, and the yields of the yeast cells showed 23.2mg/ml of culture medium.
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